IDENTIFICATION OF THE astA GENE IN ENTEROTOXIGENIC ESCHERICHIA COLI STRAINS RESPONSIBLE FOR DIARRHEA IN PIGS
نویسنده
چکیده
The astA gene responsible for production of enteroaggregative Escherichia coli heatstable enterotoxin 1 (EAST1) was identified in E. coli strains isolated from pigs with postweaning diarrhea. Two hundred and twenty-six isolates were tested using PCR for the astA marker as well as for heat-labile I (LTI), heat-stable I (STI), and heat-stable II (STII) enterotoxin genes characteristic for enterotoxigenic E. coli (ETEC). The astA-positive isolates were also analysed for fimbrial adhesins using the slide agglutination test and PCR (F18 fimbriae). It was shown that 115 (50.9 per cent) of the isolates possessed the astA gene encoding the EAST1 toxin. A close correlation between the presence of F4 fimbriae and the EAST1 gene was observed: 106 of 115 (92.2%) astA isolates expressed the F4 antigen. The results of the present study indicate that EAST1 enterotoxin may represent an additional virulence marker playing a role in the pathogenesis of porcine colibacillosis.
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